#Calcusyn serial serial#
made by serial dilutions of recombinant p24 protein (NIBSC. Mixed drug treatment is becoming common in the treatment of cancer, AIDS etc, and CalcuSyn is the most widely used software for establishing efficacy in this field. CalcuSyn performs multiple drug dose-effect calculations using the Median Effect methods described by T-C Chou and P. The use of combined drug treatments is becoming common in the treatment of cancer and AIDS. Y error bars indicate mean ± S.E.M *** p < 0. Keywords: o-(acetoxyphenyl)hept-2-ynyl sulphide, Calcusyn, peripheral blood mononuclear. CalcuSyn is the definitive analyzer of combined drug effects, able automatically to quantify phenomena such as synergism and inhibition. CalcuSyn Version 2.0 is the analyzer of combined drug effects, able automatically to quantify phenomena such as synergism and inhibition. e IC50 distribution of trabectedin as a single agent and in combination with olaparib or veliparib obtained by Calcusyn software after 72-h treatment with serial dilution of trabectedin (2–0.125 nM), olaparib (20–1.25 μM), veliparib (80–5 μM) as single agents or in constant combination with trabectedin, dashed lines indicate median IC50. FACS analysis of ( a), DNA content (cell cycle distribution) after 48-h treatment with 0.125 nM trabectedin and 1.25 μM olaparib alone and their combination, green, yellow, and light blue represent G0/G1, S phase, and G2/M phase, respectively as calculated by Flow Jo software b apoptosis (Annexin V-PI staining) obtained after 72-h treatment with 0.125 nM trabectedin and 1.25 μM olaparib alone and their combination c representative 7-day colony growth assay with 402.91 and SJSA-1 cells testing 0.125 nM trabectedin, 1.25 μM olaparib and their combination d quantitation of three independent experiments of colony growth with 402.91 and SJSA-1 cells testing trabectedin, olaparib and their combination. Biosoft: Software for Science Version 2 US 300, UK 175 single user license online fulfillment Dose-Effect Analyzer for.
#Calcusyn serial manual#
Details on the use of CalcuSyn are found in the software user's manual (14).
And values for assessing drug interactions described below, CalcuSyn can deter. The synergistic antitumor activity of trabectedin and PARP inhibitors was cell line-dependent. Calcusyn User Manual Average ratng: 9,8/10 9688 reviews.